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木豆抗旱相关基因CcGST1克隆与表达分析(PDF)

《西南林业大学学报》[ISSN:2095-1914/CN:53-1218/S]

期数:
2017年04期
页码:
1-7
栏目:
出版日期:
2017-06-30

文章信息/Info

Title:
Cloning and Expression Analysis of the Glutathione S-transferase Gene (CcGST1) Related to Drought from Cajanus cajan
文章编号:
2095-1914(2017)04-0001-07
作者:
乔光文晓鹏洪怡
贵州大学农业生物工程研究院,山地植物资源保护与种质创新省部共建教育部重点实验室,山地生态与农业生物工程协同创新中心,贵州 贵阳 550025
Author(s):
Qiao Guang Wen Xiaopeng Hong Yi
Collabrative Innovation Center for Mountain Ecology & Agro-bioengineering (CICEAB), Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-bioengineering, Guizhou University, Guizhou Guiyang 550025, China
关键词:
木豆谷胱甘肽转移酶克隆基因生物信息学表达分析
Keywords:
Cajanus cajan glutathione S-transferase (GST) colone gene bioinformatics expression analysis
分类号:
S722.36
DOI:
10.11929/j.issn.2095-1914.2017.04.001
文献标识码:
A
摘要:
为探讨谷胱甘肽转移酶 (GST) 的抗逆机理,克隆木豆中的抗旱谷胱甘肽转移酶基因,基于木豆干旱胁迫cDNA文库中1个GST基因相关的EST片段,利用RACE技术从木豆幼苗中克隆了该基因,命名为CcGST1,研究其在不同组织及不同干旱时期的表达特性。结果表明:该基因含1个669bp的开放阅读框,编码223个氨基酸残基,含有保守的谷胱甘肽-S-转移酶的N端和C端结构域;系统进化树分析结果表明木豆CcGST1与大豆、宽叶菜豆等豆科植物的GST亲缘关系较近;亚细胞定位预测CcGST1蛋白可能定位于叶绿体;荧光定量PCR分析表明,CcGST1在木豆幼苗根、茎和叶中均有表达,但根中的表达水平最高,同时该基因受干旱胁迫诱导表达。据此推测木豆CcGST1基因可能与木豆应激干旱胁迫相关。
Abstract:
One glutathione S.transferase (GST) gene associated with the drought responsiveness was cloned from Cajanus cajan to explore the stress resistance mechanism by rapid amplification of cDNA ends. It was designated CcGST1 and contained an open reading frame of 669bp, which encodes 223 amino acid residues. It also contained conserved glutathione S.transferase N- and C-terminal domain. Phylogenetic analysis revealed that CcGST1 was closely related to GST from other legume plants, such as Glycine max and Phaseolus aculifolius. Subcellular localization analysis indicated that CcGST1 was targeted to the chloroplast. By fluorescent quantitative real-time PCR, CcGST1 mRNA transcripts were detectable in all tissues, with the highest transcriptional level found in the root. Furthermore, the expression of CcGST1 transcript was markedly induced by drought stimulation. The result suggested that the CcGST1 might be involved in drought response in C.cajan.

参考文献/References

[1]唐军, 王文强, 黄春琼, 等. 木豆育种及分子生物学研究进展[J]. 热带农业科学, 2013, 33(8): 36-41.
[2]纪中华, 杨艳鲜, 拜得珍, 等. 木豆在干热河谷退化山地的生态适应性研究[J]. 干旱地区农业研究, 2007, 25(3): 158-202.
[3]郭蓓, 金文林, 赵波, 等. 木豆种质资源遗传多样性的分析[J]. 中国农学通报, 2010, 26(19): 378-382.
[4]Qiao G, Wen X P, Yu L F, et al. The enhancement of drought tolerance for pigeon pea inoculated by arbuscular mycorrhizae fungi[J]. Plant Soil and Environment, 2011, 57: 541-546.
[5]Qiao G, Wen X P, Yu L F, et al. Identification of differentially expressed genes preferably related to drought response in pigeon pea (Cajanus cajan) inoculated by arbuscular mycorrhizae fungi (AMF) [J]. Acta Physiologiae Plantarum, 2012, 34: 1711-1721.
[6]Rezaei M K, Shobbar Z S, Shahbazi M, et al. Glutathione Stransferase (GST) family in barley: Identification of members, enzyme activity, and gene expression pattern[J]. Journal of Plant Physiology, 2013, 170(14): 1277-1284.
[7]Marrs K A. The functions and regulation of glutathione S transferases in plants[J]. Annual Review of Plant Physiology & Plant Molecular Biology, 1996, 47: 127-58.
[8]胡廷章, 周大祥, 罗凯. 植物谷胱甘肽转移酶的结构与功能及其基因表达[J]. 植物生理学通讯, 2007, 43(1): 195-200.
[9]Xu J, Xing X J, Tian Y S, et al. Transgenic arabidopsis plants expressing tomato glutathione Stransferase showed enhanced resistance to salt and drought stress[J]. Plos One, 2015, 10: e0136960.
[10]George S, Venkataraman G, Parida A. A chloroplastlocalized and auxininduced glutathione Stransferase from phreatophyte Prosopis juliflora confer drought tolerance on tobacco[J]. Journal of Plant Physiology, 2010, 167(4): 311-318.
[11]Yang G, Xu Z, Peng S, et al. In planta characterization of a tau class glutathione Stransferase gene from Juglans regia (JrGSTTau1) involved in chilling tolerance[J]. Plant Cell Reports, 2016, 35(3): 681-692.
[12]杨霞, 王笑, 管荣展, 等. 多抗性稗草中2个谷胱甘肽转移酶基因的克隆与分析[J]. 江苏农业学报, 2015, 31(6): 1296-1303.
[13]徐晓俞, 李爱萍, 郑菲艳, 等. 木豆在南方水土流失治理中的作用及发展对策[J]. 福建农业科技, 2015(9): 52-54.
[14]翟妞, 刘萍萍, 陈霞, 等. 烟草谷胱甘肽转移酶基因NtGSTU17的鉴定及表达特性分析[J]. 烟草科技, 2016, 49(8): 1-7.
[15]安秀红, 徐锴, 厉恩茂, 等. 苹果抗性相关的谷胱甘肽转移酶MdGSTU1的生物信息学和表达分析[J]. 中国农业科学, 2014, 47(24): 4868-4877.
[16]史倩倩, 周琳, 王雁. 云南野生黄牡丹谷胱甘肽转移酶 (GST) 基因的分离及表达分析[J]. 林业科学, 2014, 50(12): 63-72.
[17]赵海霞, 李双江, 李成磊, 等. 苦荞谷胱甘肽转移酶 (FtGST) 基因的克隆与序列分析[J]. 生物技术通报, 2012, 28(7): 70-76.
[18]吴金华, 张西平, 胡言光, 等. 小麦抗白粉病相关基因GST克隆与表达[J]. 西北植物学报, 2013, 33(1): 34-38.
[19]Nutricati E, Miceli A, Blando F, et al. Characterization of two Arabidopsis thaliana glutathioneStransferases[J]. Plant Cell Reports, 2006, 25(9): 997-1005.
[20]Dixon D P, Cummins L, Cole D J, et al. Glutathionemediated detoxification systems in plants[J]. Current Opinion in Plant Biology, 1998, 1(3): 258-266.
[21]Cottingham C K, Meredith S, Hatzios K K. Influence of chemical treatments on glutathione Stransferases of maize with activity towards metolachlor and cinnamic acid[J]. Zeitschrift Fur Naturforschung SectionCA Journal of Biosciences, 1998, 53(12): 973-979.
[22]Dixon D P, Lapthorn A, Edwards R. Plant glutathione transferases[J]. Genome Biology, 2002, 401(3): 169-186.
[23]Mittova V, Guy M, Tal M, et al. Salinity upregulates the antioxidative system in root mitochondria and peroxisomes of the wild salttolerant tomato species Lycopersicon pennellii[J]. Journal of Experimental, 2004, 55(399): 1105-1113.
[24]Thatcher L F, Carrie C, Carol R A, et al. Differential gene expression and subcellular targeting of Arabidopsis glutathione Stransferase F8 is achieved through alternative transcription start sites[J]. Journal of Biological Chemistry, 2007, 282: 28915-28928.
[25]Kwon S H, Kwon H K, Kim W, et al. Identification of salt and drought inducible glutathione Stransferase genes of hybrid poplar[J]. Asian Pacific Journal of Cancer Prevention, 2014, 41(1): 26-32.
[26]金雪花, 洪艳, 黄河, 等. 瓜叶菊谷胱甘肽转移酶基因GST的分离及表达分析[J]. 园艺学报, 2013, 40(6): 1129-1138.
[27]张岩, 胡军, 郭长虹, 等. 植物谷胱甘肽-S-转移酶的分子生物学研究进展[J]. 哈尔滨师范大学自然科学学报, 2007, 23(4): 76-79.
[28]Galle A, Csiszar J, Secenji M, et al. Glutathione transferase activity and expression patterns during filling in flag leaves of wheat genotypes differing in drought tolerance: response to water deficit[J]. Journal of Plant Physiology, 2009, 166: 1878-1891.

备注/Memo

备注/Memo:
收稿日期:2017-01-10; 修回日期:2017-02-28
基金项目:国家自然科学基金项目 (30860224) 资助;贵州省农业攻关项目 (20092076) 资助。
第1作者:乔光 (1981—),男,博士,高级实验师。研究方向:林木遗传育种。Email: 13518504594@163.com
通信作者:文晓鹏 (1965—),男,博士,教授。研究方向:林木生物技术与遗传育种。Email: xpwensc@hotmail.com
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